| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Ismailova, Diana (11)
|
VAGATR |
30.54 |
30.98 |
0.44 |
| 2 |
Rogerson, Annika R (12)
|
VAUN-V |
31.13 |
31.22 |
0.09 |
| 3 |
Czirjak, Sara E (12)
|
VAGATR |
36.48 |
34.10 |
-2.38 |
| 4 |
Dugas, Georgia K (12)
|
VAACAC |
42.78 |
34.83 |
-7.95 |
| 5 |
McCardle, Reese B (11)
|
VAACAC |
42.94 |
36.22 |
-6.72 |
| 6 |
Hoang, Angelina (11)
|
VAACAC |
|
37.43 |
- |
| 7 |
Segura, Mara J (11)
|
VATSU |
43.36 |
38.61 |
-4.75 |
| 8 |
Turner, Paige Y (11)
|
VATSU |
41.77 |
39.39 |
-2.38 |
| 9 |
Hernandez, Maya G (11)
|
VABASS |
|
40.79 |
- |
| 10 |
Semmelhack, Sylvie W (11)
|
VAGATR |
|
44.97 |
- |
| 11 |
Brushwood, Madison P (11)
|
VABASS |
44.28 |
45.13 |
0.85 |
| 12 |
Barclay, Sydney M (12)
|
VAACAC |
43.23 |
45.20 |
1.97 |
| 13 |
Wells, Abigail J (11)
|
VATSU |
|
45.56 |
- |
| 14 |
Brooks, Madelyne G (11)
|
VABASS |
59.83 |
46.21 |
-13.62 |
| 15 |
McCORKLE, Madeline G (11)
|
VATSU |
|
47.17 |
- |
| 16 |
Mejeur, Hannah J (12)
|
VATSU |
|
1:04.14 |
- |
| 17 |
Martin, Grace E (11)
|
VABASS |
|
1:12.19 |
- |